Rf value in tlc
- how to calculate rf in tlc
- how to find rf in tlc
- how to calculate rf in thin layer chromatography
- how to calculate rf value in tlc plate
What does rf value tell you...
The Rf value is the retention factor used in the identification of organic compounds in a mixture. The Rf value is calculated by measuring the relative distance traveled by a particular organic compound with respect to the mobile phase.
It is calculated in both paper chromatography and TLC.
Thin layer chromatography (TLC) is a separation method involved in the separation of two or more organic compounds by their distribution between solid and liquid phases.
What does a high rf value mean in chromatography
The stationary (solid) phase is a polar substance in TLC while the mobile (liquid) phase is a single or combination of solvents. The absorbent is coated as a thin layer in a glass slide. The organic compounds are separated by manipulating the mobile phase.
Key Areas Covered
1.
What is TLC
– Definition, Principle, Uses
2. How to Calculate Rf Values for TLC
– Calculation of Rf Value
Key Terms: Mobile Phase, Mobility, Rf Value, Stationary Phase, Separation, Thin Layer Chromatography (TLC)
What is TLC
TLC is a chromatography technique responsible for the separation of organic compounds on
- how to calculate rf factor in tlc
- how to find rf value in tlc